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Applied and Environmental Microbiology, May 2008, p. 3030-3037, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.02760-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Dynamics of Legionella spp. and Bacterial Populations during the Proliferation of L. pneumophila in a Cooling Tower Facility{triangledown}

Nathalie Wéry,1* Valérie Bru-Adan,1 Céline Minervini,2 Jean-Philippe Delgénes,1 Laurent Garrelly,2 and Jean-Jacques Godon1

INRA, UR50, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, Narbonne F-11100, France,1 Bouisson Bertrand Laboratoires, Parc Euromédecine, 778 Rue de la Croix Verte, 34 196 Montpellier, France2

Received 7 December 2007/ Accepted 24 March 2008

The dynamics of Legionella spp. and of dominant bacteria were investigated in water from a cooling tower plant over a 9-month period which included several weeks when Legionella pneumophila proliferated. The structural diversity of both the bacteria and the Legionella spp. was monitored by a fingerprint technique, single-strand conformation polymorphism, and Legionella spp. and L. pneumophila were quantified by real-time quantitative PCR. The structure of the bacterial community did not change over time, but it was perturbed periodically by chemical treatment or biofilm detachment. In contrast, the structure of the Legionella sp. population changed in different periods, its dynamics at times showing stability but also a rapid major shift during the proliferation of L. pneumophila in July. The dynamics of the Legionella spp. and of dominant bacteria were not correlated. In particular, no change in the bacterial community structure was observed during the proliferation of L. pneumophila. Legionella spp. present in the cooling tower system were identified by cloning and sequencing of 16S rRNA genes. A high diversity of Legionella spp. was observed before proliferation, including L. lytica, L. fallonii, and other Legionella-like amoebal pathogen types, along with as-yet-undescribed species. During the proliferation of L. pneumophila, Legionella sp. diversity decreased significantly, L. fallonii and L. pneumophila being the main species recovered.

* Corresponding author. Mailing address: INRA, UR50, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, Narbonne F-11100, France. Phone: 33 468 425 186. Fax: 33 468 425 160. E-mail: weryn{at}supagro.inra.fr

{triangledown} Published ahead of print on 4 April 2008.

Applied and Environmental Microbiology, May 2008, p. 3030-3037, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.02760-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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