This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chávez-Béjar, M. I.
Right arrow Articles by Gosset, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chávez-Béjar, M. I.
Right arrow Articles by Gosset, G.
Agricola
Right arrow Articles by Chávez-Béjar, M. I.
Right arrow Articles by Gosset, G.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2008, p. 3284-3290, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.02456-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Metabolic Engineering of Escherichia coli for L-Tyrosine Production by Expression of Genes Coding for the Chorismate Mutase Domain of the Native Chorismate Mutase-Prephenate Dehydratase and a Cyclohexadienyl Dehydrogenase from Zymomonas mobilis{triangledown}

María I. Chávez-Béjar,1 Alvaro R. Lara,2,{dagger} Hezraí López,1 Georgina Hernández-Chávez,1 Alfredo Martinez,1 Octavio T. Ramírez,2 Francisco Bolívar,1 and Guillermo Gosset1*

Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Morelos 62210, México,1 Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Morelos 62210, México2

Received 31 October 2007/ Accepted 8 March 2008

The expression of the feedback inhibition-insensitive enzyme cyclohexadienyl dehydrogenase (TyrC) from Zymomonas mobilis and the chorismate mutase domain from native chorismate mutase-prephenate dehydratase (PheACM) from Escherichia coli was compared to the expression of native feedback inhibition-sensitive chorismate mutase-prephenate dehydrogenase (CM-TyrAp) with regard to the capacity to produce L-tyrosine in E. coli strains modified to increase the carbon flow to chorismate. Shake flask experiments showed that TyrC increased the yield of L-tyrosine from glucose (YL-Tyr/Glc) by 6.8-fold compared to the yield obtained with CM-TyrAp. In bioreactor experiments, a strain expressing both TyrC and PheACM produced 3 g/liter of L-tyrosine with a YL-Tyr/Glc of 66 mg/g. These values are 46 and 48% higher than the values for a strain expressing only TyrC. The results show that the feedback inhibition-insensitive enzymes can be employed for strain development as part of a metabolic engineering strategy for L-tyrosine production.

* Corresponding author. Mailing address: Instituto de Biotecnología, Universidad Nacional Autónoma de México (U.N.A.M.), Apdo. Postal 510-3, Cuernavaca, Morelos 62210, México. Phone: 52-777-3291601. Fax: 52-777-3172388. E-mail: gosset{at}ibt.unam.mx

{triangledown} Published ahead of print on 14 March 2008.

{dagger} Present address: Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Cuajimalpa, México City, México.

Applied and Environmental Microbiology, May 2008, p. 3284-3290, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.02456-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Li, P.-P., Liu, Y.-J., Liu, S.-J. (2009). Genetic and biochemical identification of the chorismate mutase from Corynebacterium glutamicum. Microbiology 155: 3382-3391 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»