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Applied and Environmental Microbiology, May 2008, p. 3295-3301, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.02507-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Assessment of the Genetic Diversity of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans as a Basis To Identify Putative Pathogenicity Genes and a Type III Secretion System of the SPI-1 Family by Multiple Suppression Subtractive Hybridizations{triangledown}

Seyed Mehdi Alavi,1 Saeideh Sanjari,1 Fabien Durand,1 Chrystelle Brin,1 Charles Manceau,1,{dagger}* and Stéphane Poussier2,{dagger}*

UMR PaVé, INRA, 42 rue Georges Morel, 49071 Beaucouzé Cedex 1, France,1 INH, 2 rue Le Nôtre, 49045 Angers Cedex 1, France2

Received 7 November 2007/ Accepted 12 March 2008

Fluorescent amplified fragment length polymorphism revealed that strains of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans are genetically distinct and can be grouped into four genetic lineages. Four suppression subtractive hybridizations were then performed to isolate DNA fragments present in these bean pathogens and absent from closely related xanthomonads. Virulence gene candidates were identified such as homologs of hemagglutinins, TonB-dependent receptors, zinc-dependent metalloproteases, type III effectors, and type IV secretion system components. Unexpectedly, homologs of the type III secretion apparatus components (SPI-1 family), usually reported in animal pathogens and insect symbionts, were also detected.

* Corresponding author. Mailing address for Stéphane Poussier: UMR PaVé, INH, 2 rue Le Nôtre, 49045 Angers Cedex 1, France. Phone: 33 241 22 55 01. Fax: 33 241 22 54 78. E-mail: stephane.poussier{at}inh.fr. Mailing address for Charles Manceau: UMR PaVé, centre INRA, 42 rue Georges Morel, 49071 Beaucouzé Cedex 1, France. Phone: 33 241 22 57 40. Fax: 33 241 22 57 05. E-mail: manceau{at}angers.inra.fr

{triangledown} Published ahead of print on 21 March 2008.

{dagger} These authors contributed equally to this work.

Applied and Environmental Microbiology, May 2008, p. 3295-3301, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.02507-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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