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Applied and Environmental Microbiology, May 2008, p. 3306-3309, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.02082-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Rapid Species Identification of Cooked Poisonous Mushrooms by Using Real-Time PCR{triangledown}

Kazuhiko Maeta,1,3 Tomoya Ochi,2 Keisuke Tokimoto,4 Norihiro Shimomura,2 Nitaro Maekawa,2 Nobuhisa Kawaguchi,5 Makoto Nakaya,6 Yutaka Kitamoto,7 and Tadanori Aimi2*

The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-cho Minami, Tottori-shi, Tottori 680-8553, Japan,1 Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori-shi, Tottori 680-8553, Japan,2 Scientific Crime Investigation Laboratory, Tottori Police Headquarters, 2-12 Chiyomi, Tottori 680-0911, Japan,3 The Tottori Mycological Institute, 211 Kokoge, 689-1125 Tottori, Japan,4 Laboratory, Biological Business Department, Ichimasa Kamaboko Co., Ltd., 77-1 Junishin, Agano-shi, Niigata 959-1936, Japan,5 Hokkaido Forest Products Research Institute, Nishikagura, Asahikawa, Hokkaido 071-0198, Japan,6 Science Laboratory, Asano Industry Co. Ltd., 3-20-6 Tamahara, Tamano, Okayama 706-0014, Japan7

Received 12 September 2007/ Accepted 21 March 2008

Species-specific identification of the major cooked and fresh poisonous mushrooms in Japan was performed using a real-time PCR system. Specific fluorescence signals were detected, and no nonspecific signals were detected. Therefore, we succeeded in developing a species-specific test for the identification of poisonous mushrooms within 1.5 h.

* Corresponding author. Mailing address: Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 690-8553, Japan. Phone and fax: 81-857-31-5372. E-mail: taimi{at}muses.tottori-u.ac.jp

{triangledown} Published ahead of print on 31 March 2008.

Applied and Environmental Microbiology, May 2008, p. 3306-3309, Vol. 74, No. 10
0099-2240/08/$08.00+0     doi:10.1128/AEM.02082-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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