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Applied and Environmental Microbiology, June 2008, p. 3387-3393, Vol. 74, No. 11
0099-2240/08/$08.00+0     doi:10.1128/AEM.02866-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Characterization of the RND-Type Multidrug Efflux Pump MexAB-OprM of the Plant Pathogen Pseudomonas syringae{triangledown}

Savina O. Stoitsova, Yvonne Braun, Matthias S. Ullrich, and Helge Weingart*

School of Engineering and Science, Jacobs University Bremen, Bremen, Germany

Received 19 December 2007/ Accepted 28 March 2008

In gram-negative bacteria, transporters belonging to the RND family are the transporters most relevant for resistance to antimicrobial compounds. In Pseudomonas aeruginosa, a clinically important pathogen, the RND-type pump MexAB-OprM has been recognized as one of the major multidrug efflux systems. Here, homologues of MexAB-OprM in the plant pathogens Pseudomonas syringae pv. phaseolicola 1448A, P. syringae pv. syringae B728a, and P. syringae pv. tomato DC3000 were identified, and mexAB-oprM-deficient mutants were generated. Determination of MICs revealed that mutation of MexAB-OprM dramatically reduced the tolerance to a broad range of antimicrobials. Moreover, the ability of the mexAB-oprM-deficient mutants to multiply in planta was reduced. RNA dot blot hybridization revealed growth-dependent regulation of the mexAB-oprM operon in P. syringae; the expression of this operon was maximal in early exponential phase and decreased gradually during further growth.

* Corresponding author. Mailing address: School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany. Phone: 49-421-2003581. Fax: 49-421-2003249. E-mail: h.weingart{at}jacobs-university.de

{triangledown} Published ahead of print on 4 April 2008.

Applied and Environmental Microbiology, June 2008, p. 3387-3393, Vol. 74, No. 11
0099-2240/08/$08.00+0     doi:10.1128/AEM.02866-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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