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Applied and Environmental Microbiology, June 2008, p. 3591-3595, Vol. 74, No. 11
0099-2240/08/$08.00+0     doi:10.1128/AEM.00098-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Coenzyme F420-Dependent Sulfite Reductase-Enabled Sulfite Detoxification and Use of Sulfite as a Sole Sulfur Source by Methanococcus maripaludis{triangledown}

Eric F. Johnson1 and Biswarup Mukhopadhyay1,2,3*

Virginia Bioinformatics Institute,1 Departments of Biochemistry,2 Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, Virginia3

Received 12 January 2008/ Accepted 19 March 2008

Coenzyme F420-dependent sulfite reductase (Fsr) of Methanocaldococcus jannaschii, a sulfite-tolerant methanogen, was expressed with activity in Methanococcus maripaludis, a sulfite-sensitive methanogen. The recombinant organism reduced sulfite to sulfide and grew with sulfite as the sole sulfur source, indicating that Fsr is a sulfite detoxification and assimilation enzyme for methanogens and that M. maripaludis synthesizes siroheme.

* Corresponding author. Mailing address: Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Washington Street 0477, Blacksburg, VA 24061. Phone: (540) 231-8015. Fax: (540) 231-2606. E-mail: biswarup{at}vt.edu

{triangledown} Published ahead of print on 31 March 2008.

Applied and Environmental Microbiology, June 2008, p. 3591-3595, Vol. 74, No. 11
0099-2240/08/$08.00+0     doi:10.1128/AEM.00098-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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