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Applied and Environmental Microbiology, June 2008, p. 3601-3604, Vol. 74, No. 11
0099-2240/08/$08.00+0     doi:10.1128/AEM.02576-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

High-Yield Production of Secreted Active Proteins by the Pseudomonas aeruginosa Type III Secretion System{triangledown} ,{dagger}

M. Derouazi,{ddagger} B. Toussaint, L. Quénée,{ddagger} O. Epaulard, M. Guillaume, R. Marlu, and B. Polack*

Laboratoire TIMC-IMAG GREPI-TheREx, Université Joseph Fourier-Grenoble 1 UMR CNRS 5525, IFR INSERM 130, Grenoble, France

Received 15 November 2007/ Accepted 22 March 2008

The Escherichia coli system is the system of choice for recombinant protein production because it is possible to obtain a high protein yield in inexpensive media. The accumulation of protein in an insoluble form in inclusion bodies remains a major disadvantage. Use of the Pseudomonas aeruginosa type III secretion system can avoid this problem, allowing the production of soluble secreted proteins.

* Corresponding author. Mailing address: Laboratoire TIMC-IMAG GREPI-TheREx, Batiment Jean Roget, Domaine de la Merci, 38706 La Tronche, France. Phone: 33(0) 476 765 967. Fax: 33(0) 476 765 935. E-mail: BPolack{at}chu-grenoble.fr

{triangledown} Published ahead of print on 4 April 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} M.D. and L.Q. contributed equally to this work.

Applied and Environmental Microbiology, June 2008, p. 3601-3604, Vol. 74, No. 11
0099-2240/08/$08.00+0     doi:10.1128/AEM.02576-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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