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Applied and Environmental Microbiology, June 2008, p. 3610-3614, Vol. 74, No. 11
0099-2240/08/$08.00+0     doi:10.1128/AEM.00045-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Detection of Active Butyrate-Degrading Microorganisms in Methanogenic Sludges by RNA-Based Stable Isotope Probing{triangledown} ,{dagger}

Masashi Hatamoto,1 Hiroyuki Imachi,1,2* Yuto Yashiro,1 Akiyoshi Ohashi,1,3 and Hideki Harada1,4

Department of Environmental Systems Engineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan,1 Subground Animalcule Retrieval Program, Extremobiosphere Research Center, Japan Agency for Marine-Earth Science & Technology, Yokosuka, Kanagawa 237-0061, Japan,2 Department of Social and Environmental Engineering, Hiroshima University, Higashi-Hiroshima 739-8527, Japan,3 Department of Civil Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan4

Received 7 January 2008/ Accepted 7 April 2008

Butyrate-degrading bacteria in four methanogenic sludges were studied by RNA-based stable isotope probing. Bacterial populations in the 13C-labeled rRNA fractions were distinct from unlabeled fractions, and Syntrophaceae species, Tepidanaerobacter sp., and Clostridium spp. dominated. These results suggest that diverse microbes were active in butyrate degradation under methanogenic conditions.

* Corresponding author. Mailing address: Subground Animalcule Retrieval Program, Extremobiosphere Research Center, Japan Agency for Marine-Earth Science & Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan. Phone: 81-46-867-9709. Fax: 81-46-867-9715. E-mail: imachi{at}jamstec.go.jp

{triangledown} Published ahead of print on 11 April 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, June 2008, p. 3610-3614, Vol. 74, No. 11
0099-2240/08/$08.00+0     doi:10.1128/AEM.00045-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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