This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Faye, T.
Right arrow Articles by Brede, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Faye, T.
Right arrow Articles by Brede, D. A.
Agricola
Right arrow Articles by Faye, T.
Right arrow Articles by Brede, D. A.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 2008, p. 3615-3617, Vol. 74, No. 11
0099-2240/08/$08.00+0     doi:10.1128/AEM.02870-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Construction of a Reporter Vector System for In Vivo Analysis of Promoter Activity in Propionibacterium freudenreichii{triangledown} ,{dagger}

Therese Faye, Anita Åsebø, Zhian Salehian, Thor Langsrud, Ingolf F. Nes, and Dag Anders Brede*

Laboratory of Microbial Gene Technology and Food Microbiology, Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (UMB), P.O. Box 5003, N-1432 Ås, Norway

Received 19 December 2007/ Accepted 8 April 2008

A β-galactosidase reporter system for the analysis of promoter elements in Propionibacterium freudenreichii was designed. The pTD210 in vivo reporter vector was constructed using a promoterless lacZ gene from Bifidobacterium longum cloned into the pAMT1 plasmid. The utility of the pTD210 reporter vector was demonstrated by an investigation of six predicted promoters in P. freudenreichii. The system produced accurate and reproducible measurements that facilitated both promoter identification and the quantification of promoter activities.

* Corresponding author. Mailing address: Laboratory of Microbial Gene Technology and Food Microbiology, Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (UMB), P.O. Box 5003, N-1432 Ås, Norway. Phone: 47 64 96 58 97. Fax: 47 64 94 14 65. E-mail: dag.anders.brede{at}umb.no

{triangledown} Published ahead of print on 18 April 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, June 2008, p. 3615-3617, Vol. 74, No. 11
0099-2240/08/$08.00+0     doi:10.1128/AEM.02870-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»