This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Almeida, R. P. P.
Right arrow Articles by Lopes, J. R. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Almeida, R. P. P.
Right arrow Articles by Lopes, J. R. S.
Agricola
Right arrow Articles by Almeida, R. P. P.
Right arrow Articles by Lopes, J. R. S.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 2008, p. 3690-3701, Vol. 74, No. 12
0099-2240/08/$08.00+0     doi:10.1128/AEM.02388-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Genetic Structure and Biology of Xylella fastidiosa Strains Causing Disease in Citrus and Coffee in Brazil{triangledown}

Rodrigo P. P. Almeida,1* Fernanda E. Nascimento,2 John Chau,1 Simone S. Prado,2 Chi-Wei Tsai,1 Sílvio A. Lopes,3 and Joao R. S. Lopes2

Department of Environmental Science, Policy and Management, University of California, Berkeley, California,1 Departamento de Entomologia, Fitopatologia e Zoologia Agrícola, ESALQ/USP, Piracicaba, Sao Paulo, Brazil,2 Departamento Científico, Fundecitrus, Araraquara, Sao Paulo, Brazil3

Received 23 October 2007/ Accepted 11 April 2008

Xylella fastidiosa is a vector-borne, plant-pathogenic bacterium that causes disease in citrus (citrus variegated chlorosis [CVC]) and coffee (coffee leaf scorch [CLS]) plants in Brazil. CVC and CLS occur sympatrically and share leafhopper vectors; thus, determining whether X. fastidiosa isolates can be dispersed from one crop to another and cause disease is of epidemiological importance. We sought to clarify the genetic and biological relationships between CVC- and CLS-causing X. fastidiosa isolates. We used cross-inoculation bioassays and microsatellite and multilocus sequence typing (MLST) approaches to determine the host range and genetic structure of 26 CVC and 20 CLS isolates collected from different regions in Brazil. Our results show that citrus and coffee X. fastidiosa isolates are biologically distinct. Cross-inoculation tests showed that isolates causing CVC and CLS in the field were able to colonize citrus and coffee plants, respectively, but not the other host, indicating biological isolation between the strains. The microsatellite analysis separated most X. fastidiosa populations tested on the basis of the host plant from which they were isolated. However, recombination among isolates was detected and a lack of congruency among phylogenetic trees was observed for the loci used in the MLST scheme. Altogether, our study indicates that CVC and CLS are caused by two biologically distinct strains of X. fastidiosa that have diverged but are genetically homogenized by frequent recombination.

* Corresponding author. Mailing address: Department of Environmental Science, Policy and Management, 137 Mulford Hall, University of California, Berkeley, CA 94720. Phone: (510) 642-1603. Fax: (510) 642-35438. E-mail: rodrigo{at}nature.berkeley.edu

{triangledown} Published ahead of print on 18 April 2008.

Applied and Environmental Microbiology, June 2008, p. 3690-3701, Vol. 74, No. 12
0099-2240/08/$08.00+0     doi:10.1128/AEM.02388-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»