This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sirikanchana, K.
Right arrow Articles by Mariñas, B. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sirikanchana, K.
Right arrow Articles by Mariñas, B. J.
Agricola
Right arrow Articles by Sirikanchana, K.
Right arrow Articles by Mariñas, B. J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 2008, p. 3774-3782, Vol. 74, No. 12
0099-2240/08/$08.00+0     doi:10.1128/AEM.02049-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Effect of Exposure to UV-C Irradiation and Monochloramine on Adenovirus Serotype 2 Early Protein Expression and DNA Replication{triangledown}

Kwanrawee Sirikanchana,1 Joanna L. Shisler,2 and Benito J. Mariñas1*

Center of Advanced Materials for the Purification of Water with Systems, Department of Civil and Environmental Engineering,1 Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois 618012

Received 7 September 2007/ Accepted 10 April 2008

The mechanisms of adenovirus serotype 2 inactivation with either UV light (with a narrow emission spectrum centered at 254 nm) or monochloramine were investigated by assessing the potential inhibition of two key steps of the adenovirus life cycle, namely, E1A protein synthesis and viral genomic replication. E1A early protein synthesis was assayed by using immunoblotting, while the replication of viral DNA was analyzed by using slot blotting. Disinfection experiments were performed in phosphate buffer solutions at pH 8 and room temperature (UV) or 20°C (monochloramine). Experimental results revealed that normalized E1A levels at 12 h postinfection (p.i.) were statistically the same as the corresponding decrease in survival ratio for both UV and monochloramine disinfection. Normalized DNA levels at 24 h p.i. were also found to be statistically the same as the corresponding decrease in survival ratio for monochloramine disinfection. In contrast, for UV disinfection, genomic DNA levels were much lower than E1A or survival ratios, possibly as a result of a delay in DNA replication for UV-treated virions compared to that for controls. Future efforts will determine the pre-E1A synthesis step in the adenovirus life cycle affected by exposure to UV and monochloramine, with the goal of identifying the viral molecular target of these two disinfectants.

* Corresponding author. Mailing address: Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801. Phone: (217) 333-6961. Fax: (217) 333-6968. E-mail: marinas{at}uiuc.edu

{triangledown} Published ahead of print on 18 April 2008.

Applied and Environmental Microbiology, June 2008, p. 3774-3782, Vol. 74, No. 12
0099-2240/08/$08.00+0     doi:10.1128/AEM.02049-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»