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Applied and Environmental Microbiology, June 2008, p. 3783-3794, Vol. 74, No. 12
0099-2240/08/$08.00+0     doi:10.1128/AEM.02302-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Molecular Analysis of the Enterobacter sakazakii O-Antigen Gene Locus{triangledown}

N. Mullane,1 P. O'Gaora,2 J. E. Nally,3 C. Iversen,4 P. Whyte,1 P. G. Wall,5 and S. Fanning1*

Centres for Food Safety and Food-borne Zoonomics, UCD Veterinary Sciences Centre,1 School of Agriculture, Food Science & Veterinary Medicine,3 Conway Institute of Biomolecular & Biomedical Research,2 School of Public Health & Population Sciences, University College Dublin, Belfield, Dublin 4, Ireland,5 Institute for Food Safety & Hygiene, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, Switzerland4

Received 11 October 2007/ Accepted 16 April 2008

Nucleotide polymorphism associated with the O-antigen-encoding locus, rfb, in Enterobacter sakazakii was determined by PCR-restriction fragment length polymorphism analysis. Based on the analysis of these DNA profiles, 12 unique banding patterns were detected among a collection of 62 strains from diverse origins. Two common profiles were identified and were designated serotypes O:1 and O:2. DNA sequencing of the 12,500-bp region flanked by galF and gnd identified 11 open reading frames, all with the same transcriptional direction. Analysis of the proximal region of both sequences demonstrated remarkable heterogeneity. A PCR assay targeting genes specific for the two prominent serotypes was developed and applied for the identification of these strains recovered from food, environmental, and clinical samples.

* Corresponding author. Mailing address: Centres for Food Safety & Food-borne Zoonomics, UCD Veterinary Sciences Centre, University College Dublin, Belfield, Dublin 4, Ireland. Phone: (353-1) 716 6082. Fax: (353-1) 716 6091. E-mail: sfanning{at}ucd.ie

{triangledown} Published ahead of print on 25 April 2008.

Applied and Environmental Microbiology, June 2008, p. 3783-3794, Vol. 74, No. 12
0099-2240/08/$08.00+0     doi:10.1128/AEM.02302-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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