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Applied and Environmental Microbiology, June 2008, p. 3908-3911, Vol. 74, No. 12
0099-2240/08/$08.00+0     doi:10.1128/AEM.02928-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted{triangledown}

Emma R. Master,1,{ddagger},{dagger} Nathalie Y. R. Agar,2,§,{dagger} Leticia Gómez-Gil,1,¶ Justin B. Powlowski,2 William W. Mohn,1 and Lindsay D. Eltis1*

Department of Microbiology and Immunology, Life Sciences Centre, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada,1 Department of Chemistry and Biochemistry, Concordia University, Montreal, Quebec H4B 1R6, Canada2

Received 27 December 2007/ Accepted 14 April 2008

Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDOCam-1) was purified and found to have an apparent Formula for biphenyl of 1.1 ± 0.1 s–1 (mean ± standard deviation) at 4°C. In contrast, BPDOLB400 from the mesophile Burkholderia xenovorans LB400 had no detectable activity at this temperature. At 57°C, the half-life of the BPDOCam-1 oxygenase was less than half that of the BPDOLB400 oxygenase. Nevertheless, BPDOCam-1 appears to be a typical Pseudomonas pseudoalcaligenes KF707-type dioxygenase.

* Corresponding author: Department of Microbiology and Immunology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada. Phone: (604) 822-0042. Fax: (604) 822-6041. E-mail: leltis{at}interchange.ubc.ca

{triangledown} Published ahead of print on 18 April 2008.

{ddagger} Present address: Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario M5S 3E5, Canada.

{dagger} These authors contributed equally to this paper.

§ Present address: Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.

Present address: Structure and Function of Biological Membranes, Université Libre de Bruxelles, 1050 Brussels, Belgium.

Applied and Environmental Microbiology, June 2008, p. 3908-3911, Vol. 74, No. 12
0099-2240/08/$08.00+0     doi:10.1128/AEM.02928-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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