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Applied and Environmental Microbiology, June 2008, p. 3912-3914, Vol. 74, No. 12
0099-2240/08/$08.00+0     doi:10.1128/AEM.00127-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Rapid and Sensitive Detection of Norovirus Genomes in Oysters by a Two-Step Isothermal Amplification Assay System Combining Nucleic Acid Sequence-Based Amplification and Reverse Transcription-Loop-Mediated Isothermal Amplification Assays{triangledown}

Shinji Fukuda,* Yukie Sasaki, and Masato Seno

Center for Public Health and Environment, Hiroshima Prefectural Technology Research Institute, Hiroshima 734-0007, Japan

Received 15 January 2008/ Accepted 22 April 2008

We developed a two-step isothermal amplification assay system, which achieved the detection of norovirus (NoV) genomes in oysters with a sensitivity similar to that of reverse transcription-seminested PCR. The time taken for the amplification of NoV genomes from RNA extracts was shortened to about 3 h.

* Corresponding author. Mailing address: Center for Public Health and Environment, Hiroshima Prefectural Technology Research Institute, Minami-machi 1-6-29, Minami-ku, Hiroshima 734-0007, Japan. Phone: 81 82 255 7142. Fax: 81 82 252 8642. E-mail: s-fukuda80723{at}pref.hiroshima.lg.jp

{triangledown} Published ahead of print on 2 May 2008.

Applied and Environmental Microbiology, June 2008, p. 3912-3914, Vol. 74, No. 12
0099-2240/08/$08.00+0     doi:10.1128/AEM.00127-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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