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Applied and Environmental Microbiology, July 2008, p. 4144-4148, Vol. 74, No. 13
0099-2240/08/$08.00+0     doi:10.1128/AEM.00376-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Phosphorus Availability Influences Elemental Uptake in the Mycorrhizal Fungus Glomus intraradices, as Revealed by Particle-Induced X-Ray Emission Analysis{triangledown}

Pål Axel Olsson,1* Edith C. Hammer,2 Håkan Wallander,2 and Jan Pallon3

Department of Plant Ecology and Systematics,1 Department of Microbial Ecology,2 Department of Nuclear Physics, Lund University, Lund, Sweden3

Received 14 February 2008/ Accepted 4 May 2008

We investigated element accumulation in the arbuscular mycorrhizal fungus Glomus intraradices. Fungal spores and mycelia growing in monoxenic cultures were analyzed. The elemental composition was quantified using particle-induced X-ray emission (PIXE) in combination with scanning transmission ion microscopy. In the spores, Ca and Fe were associated mainly with the spore wall, while P and K showed patchy distributions and their concentrations were correlated. Excess of P in the hyphal growth medium increased the P and Si concentrations in spores and increased the K/Ca ratio in spores. Increased P availability decreased the concentration of Zn and Mn in spores. We concluded that the availability of P influences the uptake and accumulation of several elements in spores. It is demonstrated that PIXE analysis is a powerful tool for quantitative analysis of elemental accumulation in fungal mycelia.

* Corresponding author. Mailing address: Department of Plant Ecology and Systematics, Ecology Building, Lund University, SE-223 62 Lund, Sweden. Phone: 46 46 222 4247. Fax: 46 46 222 4158. E-mail: pal_axel.olsson{at}ekol.lu.se

{triangledown} Published ahead of print on 9 May 2008.

Applied and Environmental Microbiology, July 2008, p. 4144-4148, Vol. 74, No. 13
0099-2240/08/$08.00+0     doi:10.1128/AEM.00376-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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