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Applied and Environmental Microbiology, July 2008, p. 4226-4230, Vol. 74, No. 13
0099-2240/08/$08.00+0     doi:10.1128/AEM.00013-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Immunomagnetic Separation Combined with Real-Time Reverse Transcriptase PCR Assays for Detection of Norovirus in Contaminated Food{triangledown}

YoungBin Park,1 You-Hee Cho,3 YoungMee Jee,4 and GwangPyo Ko1,2*

Department of Environmental Health and Institute of Health and Environment, School of Public Health, Seoul National University, Seoul, Korea,1 Institute of Microbiology, School of Biological Sciences, Seoul National University, Seoul, Korea,2 Department of Life Science, Sogang University, Seoul, Korea,3 Division of Enteric and Hepatitis Viruses, Center for Infectious Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul, Korea4

Received 3 January 2008/ Accepted 19 April 2008

We developed an immunomagnetic separation (IMS) technique combined with real-time TaqMan reverse transcriptase PCR (RT-PCR), which allowed detection of norovirus at a level as low as 3 to 7 RT-PCR units from artificially contaminated strawberries. The inoculum recovery rate ranged from 14 to 30%. The data demonstrate that IMS combined with real-time RT-PCR will be useful as a rapid and sensitive method for detecting food-borne microbial contaminants.

* Corresponding author. Mailing address: Department of Environmental Health, School of Public Health, Seoul National University, 28 Yunkeun-Dong, Chongro-Ku, Seoul 110-799, South Korea. Phone: 82-2-3668-7881. Fax: 82-2-745-9104. E-mail: gko{at}snu.ac.kr

{triangledown} Published ahead of print on 25 April 2008.

Applied and Environmental Microbiology, July 2008, p. 4226-4230, Vol. 74, No. 13
0099-2240/08/$08.00+0     doi:10.1128/AEM.00013-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Morton, V., Jean, J., Farber, J., Mattison, K. (2009). Detection of Noroviruses in Ready-To-Eat Foods by Using Carbohydrate-Coated Magnetic Beads. Appl. Environ. Microbiol. 75: 4641-4643 [Abstract] [Full Text]  
  • Cannon, J. L., Vinje, J. (2008). Histo-Blood Group Antigen Assay for Detecting Noroviruses in Water. Appl. Environ. Microbiol. 74: 6818-6819 [Abstract] [Full Text]  


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