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Applied and Environmental Microbiology, July 2008, p. 4231-4235, Vol. 74, No. 13
0099-2240/08/$08.00+0     doi:10.1128/AEM.02545-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Use of Laser Microdissection for Phylogenetic Characterization of Polyphosphate-Accumulating Bacteria{triangledown}

Stefanie Gloess,1 Hans-Peter Grossart,1 Martin Allgaier,1,{dagger} Stefan Ratering,2* and Michael Hupfer1*

Leibniz Institute of Freshwater Ecology and Inland Fisheries, Alte Fischerhütte 2, D-16775 Stechlin-Neuglobsow, Germany,1 Institute of Applied Microbiology, Justus-Liebig University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany2

Received 12 November 2007/ Accepted 22 April 2008

Our novel approach for taxonomic identification of uncultured bacteria harboring specific physiological features in complex environmental samples combines cell collection by laser microdissection and subsequent DNA analysis. The newly developed approach was successfully tested for collection and phylogenetic characterization of polyphosphate-accumulating bacteria in activated sludge and lake sediment.

* Corresponding author. Mailing address for Stefan Ratering (microdissection): Institute of Applied Microbiology, Justus-Liebig University Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany. Phone: 49 641 9937354. Fax: 49 641 9937359. E-mail: Stefan.Ratering{at}agrar.uni-giessen.de. Mailing address for Michael Hupfer (poly-P bacteria): Leibniz Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, D-12587 Berlin, Germany. Phone: 49 30 64181 605. Fax: 49 30 64181682. E-mail: hupfer{at}igb-berlin.de

{triangledown} Published ahead of print on 2 May 2008.

{dagger} Present address: DOE Joint Genome Institute, 2800 Mitchell Dr., Walnut Creek, CA 94598.

Applied and Environmental Microbiology, July 2008, p. 4231-4235, Vol. 74, No. 13
0099-2240/08/$08.00+0     doi:10.1128/AEM.02545-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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