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Applied and Environmental Microbiology, July 2008, p. 4300-4308, Vol. 74, No. 14
0099-2240/08/$08.00+0     doi:10.1128/AEM.00864-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Presence of a Family of Plasmids (29 to 65 Kilobases) with a 26-Kilobase Common Region in Different Strains of the Sulfur-Oxidizing Bacterium Acidithiobacillus caldus{triangledown} ,{dagger}

Leonard J. van Zyl, Shelly M. Deane, Lilly-Ann Louw, and Douglas E. Rawlings*

Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa

Received 16 April 2008/ Accepted 21 May 2008

Three large cryptic plasmids from different isolates of Acidithiobacillus caldus were rescued by using an in vitro transposition system that delivers a kanamycin-selectable marker and an Escherichia coli plasmid origin of replication. The largest of the plasmids, the 65-kb plasmid pTcM1, was isolated from a South African A. caldus strain, MNG. This plasmid was sequenced and compared to that of pTcF1 (39 kb, from strain "f," South Africa) and pC-SH12 (29 kb, from strain C-SH12, Australia). With the exception of a 2.7-kb insertion sequence, pC-SH12 appears to represent the DNA common to all three plasmids and includes a number of accessory genes plus the plasmid "backbone" containing the replication region. The two larger plasmids carry, in addition, a number of insertion sequences of the ISL3 family and a composite transposon related to the Tn21 subfamily containing a highly mosaic region within the borders of the inverted repeats. Genes coding for arsenic resistance, plasmid mobilization, plasmid stability, and a putative restriction-modification system occur within these mosaic regions.

* Corresponding author. Mailing address: Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa. Phone: 27-21-808 3071. Fax: 27-21-808 3680. E-mail: der{at}sun.ac.za

{triangledown} Published ahead of print on 30 May 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, July 2008, p. 4300-4308, Vol. 74, No. 14
0099-2240/08/$08.00+0     doi:10.1128/AEM.00864-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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