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Applied and Environmental Microbiology, July 2008, p. 4567-4573, Vol. 74, No. 14
0099-2240/08/$08.00+0     doi:10.1128/AEM.02851-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Diversity Surveys and Evolutionary Relationships of aoxB Genes in Aerobic Arsenite-Oxidizing Bacteria{triangledown} ,{dagger}

Marianne Quéméneur,1 Audrey Heinrich-Salmeron,2 Daniel Muller,2,3 Didier Lièvremont,2 Michel Jauzein,4 Philippe N. Bertin,2 Francis Garrido,1 and Catherine Joulian1*

BRGM, Ecotechnology Unit, Process and Environment Division, 3 Avenue Claude Guillemin, 45060 Orléans Cedex 02, France,1 Génétique Moléculaire, Génomique et Microbiologie, UMR 7156 CNRS and Université Louis Pasteur, 28 Rue Goethe, 67000 Strasbourg, France,2 Écologie Microbienne, UMR CNRS 5557 USC INRA 1193 and Université de Lyon, 69622 Villeurbanne Cedex, France,3 LIMOS, UMR 7137 CNRS and Université de Nancy, BP 239, 54506 Vandoeuvre-les-Nancy Cedex, France4

Received 18 December 2007/ Accepted 12 May 2008

A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential amino acids and structural motifs. AoxB phylogeny of pure strains showed well-discriminated taxonomic groups and was similar to 16S rRNA phylogeny. Alphaproteobacteria-, Betaproteobacteria-, and Gammaproteobacteria-related sequences were retrieved from environmental surveys, demonstrating their prevalence in mesophilic As-contaminated soils. Our study underlines the usefulness of the aoxB gene as a functional marker of aerobic As(III) oxidizers.

* Corresponding author. Mailing address: BRGM, Ecotechnology Unit, Process and Environment Division, 3 Avenue Claude Guillemin, 45060 Orléans Cedex 02, France. Phone: 33(0)2 3864 3089. Fax: 33(0)2 3864 3680. E-mail: c.joulian{at}brgm.fr

{triangledown} Published ahead of print on 23 May 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, July 2008, p. 4567-4573, Vol. 74, No. 14
0099-2240/08/$08.00+0     doi:10.1128/AEM.02851-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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