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Applied and Environmental Microbiology, July 2008, p. 4574-4579, Vol. 74, No. 14
0099-2240/08/$08.00+0     doi:10.1128/AEM.00201-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Multichromosomal Genome Structure and Confirmation of Diazotrophy in Novel Plant-Associated Burkholderia Species{triangledown}

Lourdes Martínez-Aguilar,1 Rafael Díaz,1 Juan José Peña-Cabriales,2 Paulina Estrada-de los Santos,1 Michael F. Dunn,1 and Jesús Caballero-Mellado1*

Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Ap. Postal No. 565-A, Cuernavaca, Morelos, México,1 Centro de Investigación y de Estudios Avanzados, Km 9.6 Libramiento Norte Carr. Irapuato-León, Irapuato, Guanajuato, México2

Received 22 January 2008/ Accepted 13 May 2008

Pulsed-field gel electrophoresis and 16S rRNA hybridization experiments showed that multichromosome genome structures and very large genome sizes (6.46 to 8.73 Mb) are prevalent in novel plant-associated Burkholderia species. 15N2 isotope dilution assays revealed unambiguous diazotrophy in these novel species. nifH gene sequence analysis, often used to determine phylogenetic relatedness among diazotrophs, showed tight clusters of Burkholderia species, which were clearly distinct from those of other diazotrophs.

* Corresponding author. Mailing address: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Ap. Postal 565-A, Cuernavaca, Morelos, México. Phone: (52 777) 329-17-03. Fax: (52 777) 317-55-81. E-mail: jesuscab{at}ccg.unam.mx

{triangledown} Published ahead of print on 23 May 2008.

Applied and Environmental Microbiology, July 2008, p. 4574-4579, Vol. 74, No. 14
0099-2240/08/$08.00+0     doi:10.1128/AEM.00201-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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