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Applied and Environmental Microbiology, August 2008, p. 4779-4781, Vol. 74, No. 15
0099-2240/08/$08.00+0     doi:10.1128/AEM.00295-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Multiplex Fast Real-Time PCR for Quantitative Detection and Identification of cos- and pac-Type Streptococcus thermophilus Bacteriophages{triangledown} ,{dagger}

Beatriz del Rio,{ddagger} María Cruz Martín,{ddagger} Noelia Martínez, Alfonso H. Magadán, and Miguel A. Alvarez*

Instituto de Productos Lácteos de Asturias (IPLA, CSIC), 33300 Villaviciosa, Asturias, Spain

Received 2 February 2008/ Accepted 5 February 2008

The fermentation of milk by Streptococcus thermophilus is a widespread industrial process that is susceptible to bacteriophage attack. In this work, a preventive fast real-time PCR method for the detection, quantification, and identification of types of S. thermophilus phages in 30 min is described.

* Corresponding author. Mailing address: Instituto de Productos Lácteos de Asturias (CSIC), 33300 Villaviciosa, Asturias, Spain. Phone: 34 985 89 21 31. Fax: 34 985 89 22 33. E-mail: maag{at}ipla.csic.es

{triangledown} Published ahead of print on 6 June 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} B.D.R. and M.C.M. contributed equally to this work.

Applied and Environmental Microbiology, August 2008, p. 4779-4781, Vol. 74, No. 15
0099-2240/08/$08.00+0     doi:10.1128/AEM.00295-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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