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Applied and Environmental Microbiology, August 2008, p. 4847-4852, Vol. 74, No. 15
0099-2240/08/$08.00+0     doi:10.1128/AEM.00555-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Conversion of Daidzein and Genistein by an Anaerobic Bacterium Newly Isolated from the Mouse Intestine{triangledown}

Anastasia Matthies,1,{dagger} Thomas Clavel,2,{dagger} Michael Gütschow,3 Wolfram Engst,4 Dirk Haller,2 Michael Blaut,1 and Annett Braune1*

Department of Gastrointestinal Microbiology,1 Analytics Group, German Institute of Human Nutrition Potsdam-Rehbruecke, Arthur-Scheunert-Allee 114-116, D-14558 Nuthetal, Germany,4 Chair for Biofunctionality, Nutrition and Food Research Centre, Technical University of Munich, Am Forum 5, D-85354 Freising, Germany,2 Pharmaceutical Institute, Pharmaceutical Chemistry I, University of Bonn, An der Immenburg 4, D-53121 Bonn, Germany3

Received 7 March 2008/ Accepted 27 May 2008

The metabolism of isoflavones by gut bacteria plays a key role in the availability and bioactivation of these compounds in the intestine. Daidzein and genistein are the most common dietary soy isoflavones. While daidzein conversion yielding equol has been known for some time, the corresponding formation of 5-hydroxy-equol from genistein has not been reported previously. We isolated a strictly anaerobic bacterium (Mt1B8) from the mouse intestine which converted daidzein via dihydrodaidzein to equol as well as genistein via dihydrogenistein to 5-hydroxy-equol. Strain Mt1B8 was a gram-positive, rod-shaped bacterium identified as a member of the Coriobacteriaceae. Strain Mt1B8 also transformed dihydrodaidzein and dihydrogenistein to equol and 5-hydroxy-equol, respectively. The conversion of daidzein, genistein, dihydrodaidzein, and dihydrogenistein in the stationary growth phase depended on preincubation with the corresponding isoflavonoid, indicating enzyme induction. Moreover, dihydrogenistein was transformed even more rapidly in the stationary phase when strain Mt1B8 was grown on either genistein or daidzein. Growing the cells on daidzein also enabled conversion of genistein. This suggests that the same enzymes are involved in the conversion of the two isoflavones.

* Corresponding author. Mailing address: Department of Gastrointestinal Microbiology, German Institute of Human Nutrition Potsdam-Rehbruecke, Arthur-Scheunert-Allee 114-116, D-14558 Nuthetal, Germany. Phone: 49-33200-88402. Fax: 49-33200-88407. E-mail: braune{at}dife.de

{triangledown} Published ahead of print on 6 June 2008.

{dagger} A.M. and T.C. contributed equally to this work.

Applied and Environmental Microbiology, August 2008, p. 4847-4852, Vol. 74, No. 15
0099-2240/08/$08.00+0     doi:10.1128/AEM.00555-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kim, M., Kim, S.-I., Han, J., Wang, X.-L., Song, D.-G., Kim, S.-U. (2009). Stereospecific Biotransformation of Dihydrodaidzein into (3S)-Equol by the Human Intestinal Bacterium Eggerthella Strain Julong 732. Appl. Environ. Microbiol. 75: 3062-3068 [Abstract] [Full Text]  
  • Matthies, A., Blaut, M., Braune, A. (2009). Isolation of a Human Intestinal Bacterium Capable of Daidzein and Genistein Conversion. Appl. Environ. Microbiol. 75: 1740-1744 [Abstract] [Full Text]  


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