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Applied and Environmental Microbiology, August 2008, p. 4978-4981, Vol. 74, No. 15
0099-2240/08/$08.00+0     doi:10.1128/AEM.00219-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Detection of a Lysozyme Inhibitor in Proteus mirabilis by a New Reverse Zymogram Method{triangledown}

Lien Callewaert,1 Lise Vanderkelen,1 Daphne Deckers,1 Abram Aertsen,1 Johan Robben,2,{dagger} and Chris W. Michiels1*

Laboratory of Food Microbiology and Leuven Food Science and Nutrition Research Center (LFoRCe), Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, B-3001 Leuven, Belgium,1 Biomedical Research Institute (BIOMED), Hasselt University and Transnationale Universiteit Limburg, School of Life Sciences, Agoralaan Building A, B-3590 Diepenbeek, Belgium2

Received 24 January 2008/ Accepted 28 May 2008

A reverse zymogram method for the detection of bacterial lysozyme inhibitors was developed. This method was validated by using a periplasmic protein extract of Escherichia coli containing a known inhibitor and subsequently led to the detection of a new proteinaceous hen egg white lysozyme inhibitor in Proteus mirabilis.

* Corresponding author. Mailing address: Laboratory of Food Microbiology, Kasteelpark Arenberg 22, B-3001 Leuven, Belgium. Phone: 32-16-321578. Fax: 32-16-321960. E-mail: Chris.Michiels{at}biw.kuleuven.be

{triangledown} Published ahead of print on 6 June 2008.

{dagger} Present address: Section Biochemistry, Molecular and Structural Biology, Katholieke Universiteit Leuven, Celestijnenlaan 200G, B-3001 Leuven, Belgium.

Applied and Environmental Microbiology, August 2008, p. 4978-4981, Vol. 74, No. 15
0099-2240/08/$08.00+0     doi:10.1128/AEM.00219-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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