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Applied and Environmental Microbiology, August 2008, p. 5113-5120, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.00306-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Simple Method for Plating Escherichia coli Bacteriophages Forming Very Small Plaques or No Plaques under Standard Conditions {triangledown}

Joanna M. Los,1,{dagger} Piotr Golec,1,{dagger} Grzegorz Wegrzyn,1,2 Alicja Wegrzyn,3 and Marcin Los1*

Department of Molecular Biology, University of Gdansk, Kadki 24, 80-822 Gdansk, Poland,1 Institute of Oceanology, Polish Academy of Sciences, Powstanców Warszawy 55, 81-721 Sopot, Poland,2 Laboratory of Molecular Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Kladki 24, 80-822 Gdansk, Poland3

Received 5 February 2008/ Accepted 20 June 2008

The use of low concentrations (optimally 2.5 to 3.5 µg/ml, depending on top agar thickness) of ampicillin in the bottom agar of the plate allows for formation of highly visible plaques of bacteriophages which otherwise form extremely small plaques or no plaques on Escherichia coli lawns. Using this method, we were able to obtain plaques of newly isolated bacteriophages, propagated after induction of prophages present in six E. coli O157:H– strains which did not form plaques when standard plating procedures were employed.

* Corresponding author. Mailing address: Department of Molecular Biology, University of Gdansk, Kladki 24, 80-822 Gdansk, Poland. Phone: 48 58 5236319. Fax: 48 58 5236424. E-mail: mlos{at}biotech.ug.gda.pl

{triangledown} Published ahead of print on 27 June 2008.

{dagger} J.M.L. and P.G. contributed equally to this work.

Applied and Environmental Microbiology, August 2008, p. 5113-5120, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.00306-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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