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Applied and Environmental Microbiology, August 2008, p. 5237-5240, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.00923-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Influence of Particle Size on Bacterial Community Structure in Aquatic Sediments as Revealed by 16S rRNA Gene Sequence Analysis{triangledown}

Colin R. Jackson* and Andrew Q. Weeks

Department of Biology, The University of Mississippi, University, Mississippi 38677

Received 23 April 2008/ Accepted 11 June 2008

Bacterial communities associated with sediment particles were examined using PCR-denaturing gradient gel electrophoresis and 16S rRNA gene sequencing. Particle size influenced community structure, with attached bacterial assemblages separating into 63- to 125-, 125- to 1,000-, and 1,000- to 2,000-µm fractions. Differences were particularly pronounced for the Verrucomicrobia-Planctomycetes, whose numbers were significantly reduced on coarser particles.


* Corresponding author. Mailing address: Department of Biology, Shoemaker Hall, The University of Mississippi, University, MS 38677. Phone: (662) 915-7495. Fax: (662) 915-5144. E-mail: cjackson{at}olemiss.edu

{triangledown} Published ahead of print on 20 June 2008.


Applied and Environmental Microbiology, August 2008, p. 5237-5240, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.00923-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.