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Applied and Environmental Microbiology, August 2008, p. 5241-5243, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.02580-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Denaturing Gradient Gel Electrophoresis as a Tool To Determine Batch Similarity of Probiotic Cultures of Porcine Cecal Bacteria{triangledown}

Michael E. Hume,1* Charles M. Scanlan,2 Roger B. Harvey,1 Kathleen Andrews,1 James D. Snodgrass,1 Armen G. Nalian,3 Alexandra Martynova-Van Kley,3 and David J. Nisbet1

USDA, ARS, SPARC, Food and Feed Safety Research Unit, College Station, Texas 778445,1 Department of Veterinary Pathobiology, Texas Veterinary Medical Center, Texas A&M University, College Station, Texas 77843,2 Department of Biotechnology, Stephen F. Austin State University, Nacogdoches, Texas 759653

Received 15 November 2007/ Accepted 13 June 2008

A continuous-flow porcine cecal bacterial culture has been used experimentally as treatment against enterotoxigenic Escherichia coli in weanling pigs. Periodically, the cultures must be started from frozen stock. Our results indicate that denaturing gradient gel electrophoresis can be applied as an indirect indication of culture similarity for each new batch generated from frozen stock.

* Corresponding author. Mailing address: USDA, ARS, SPARC, FFSRU, 2881 F&B Road, College Station, TX 77845. Phone: (979) 260-9404. Fax: (979) 260-9332. E-mail: Michael.Hume{at}ars.usda.gov

{triangledown} Published ahead of print on 27 June 2008.

Applied and Environmental Microbiology, August 2008, p. 5241-5243, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.02580-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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