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Applied and Environmental Microbiology, August 2008, p. 5259-5262, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.00463-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Heterologous Expression of a Position 2-Substituted (1->3)-β-D-Glucan in Lactococcus lactis{triangledown} ,{dagger}

María Laura Werning,1 María Angeles Corrales,1 Alicia Prieto,1 Pilar Fernández de Palencia,1 Jesús Navas,2 and Paloma López1*

Departamento de Ciencia de Proteínas, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain,1 Departamento de Biología Molecular (Unidad Asociada al Centro de Investigaciones Biológicas, CSIC), Facultad de Medicina, Universidad de Cantabria, 39011 Santander, Spain2

Received 26 February 2008/ Accepted 9 June 2008

Exopolysaccharides play an important role in the rheology and texture of fermented foods, and among these β-glucans have immunomodulating properties. We show that the overproduction of the Pediococcus parvulus GTF glycosyltransferase in an uncapsulated Lactococcus lactis strain results in synthesis and secretion (300 mg liter–1) of a position 2-substituted (1->3)-β-D-glucan that has potential use as a food additive.

* Corresponding author. Mailing address: Centro de Investigaciones Biológicas, Ramiro de Maeztu 9, 28040 Madrid, Spain. Phone: 34-918373112. Fax: 34-915360432. E-mail: plg{at}cib.csic.es

{triangledown} Published ahead of print on 20 June 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, August 2008, p. 5259-5262, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.00463-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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