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Applied and Environmental Microbiology, September 2008, p. 5359-5365, Vol. 74, No. 17
0099-2240/08/$08.00+0     doi:10.1128/AEM.02433-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Gene Silencing by RNA Interference in the White Rot Fungus Phanerochaete chrysosporium{triangledown}

Avi Matityahu,1,2 Yitzhak Hadar,2 Carlos G. Dosoretz,3 and Paula A. Belinky1,4*

MIGAL—Galilee Technology Center, Kiryat Shmona 11016, Israel,1 Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel,2 Division of Environmental, Water and Agricultural Engineering, Faculty of Civil and Environmental Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel,3 Tel Hai Academic College, Kiryat Shmona 12210, Israel4

Received 29 October 2007/ Accepted 23 June 2008

The effectiveness of RNA interference (RNAi) is demonstrated in the lignin-degrading fungus Phanerochaete chrysosporium. The manganese-containing superoxide dismutase gene (MnSOD1) was used as the target for RNAi. The plasmid constructed for gene silencing contained a transcriptional unit for hairpin RNA expression. Significantly lower MnSOD expression at both the mRNA and protein activity levels was detected in RNAi transformants. Furthermore, even though P. chrysosporium possesses three copies of the MnSOD gene, this RNAi construct was sufficient to decrease the enzymatic activity by as much as 70% relative to control levels. Implementation of the RNAi technique in P. chrysosporium provides an alternative genetic tool for studies of gene function, particularly of essential genes or gene families.

* Corresponding author. Mailing address: MIGAL—Galilee Technology Center, Kiryat Shmona 11016, Israel. Phone: 972-4-6953507. Fax: 972-4-6944980. E-mail: paula{at}migal.org.il

{triangledown} Published ahead of print on 7 July 2008.

Applied and Environmental Microbiology, September 2008, p. 5359-5365, Vol. 74, No. 17
0099-2240/08/$08.00+0     doi:10.1128/AEM.02433-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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