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Applied and Environmental Microbiology, September 2008, p. 5429-5435, Vol. 74, No. 17
0099-2240/08/$08.00+0     doi:10.1128/AEM.00801-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Development of a Novel Biofilm Continuous Culture Method for Simultaneous Assessment of Architecture and Gaseous Metabolite Production{triangledown}

Yutaka Yawata,{dagger} Nobuhiko Nomura,{dagger}* and Hiroo Uchiyama

Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan

Received 8 April 2008/ Accepted 27 June 2008

The way that gaseous metabolite production changes along with biofilm architecture development is poorly understood. To address this question, we developed a novel flow reactor biofilm culture method that allows for simultaneous assessment of gaseous metabolite production and architecture visualization. In this report, we establish the utility of this method using denitrification by Pseudomonas aeruginosa biofilms as a model system. Using this method, we were able to collect and analyze gaseous metabolites produced by denitrification and also visualize biofilm architecture in a nondestructive manner. Thus, we propose that this novel method is a powerful tool to investigate potential relationships between biofilm architecture and the gas-producing metabolic activity of biofilms, providing new insights into biofilm ecology.

* Corresponding author. Mailing address: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan. Phone and fax: 81-29-853-6627. E-mail: nomunobu{at}sakura.cc.tsukuba.ac.jp

{triangledown} Published ahead of print on 7 July 2008.

{dagger} Y.Y. and N.N. contributed equally to this study.

Applied and Environmental Microbiology, September 2008, p. 5429-5435, Vol. 74, No. 17
0099-2240/08/$08.00+0     doi:10.1128/AEM.00801-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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