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Applied and Environmental Microbiology, September 2008, p. 5451-5456, Vol. 74, No. 17
0099-2240/08/$08.00+0     doi:10.1128/AEM.02419-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Listeria monocytogenes Biofilm-Associated Protein (BapL) May Contribute to Surface Attachment of L. monocytogenes but Is Absent from Many Field Isolates{triangledown}

Suzanne J. Jordan,1 Stefano Perni,4 Sarah Glenn,1 Isabel Fernandes,2 Manuela Barbosa,2 Manuela Sol,2 Rogerio P. Tenreiro,3 Lelia Chambel,3 Belarmino Barata,3 Isabel Zilhao,3 Timothy G. Aldsworth,4 Andreia Adriao,5 M. Leonor Faleiro,5 Gilbert Shama,4 and Peter W. Andrew1*

Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, United Kingdom,1 INETI-DTIA, EdificoS, Estrada do Paco Lumiar, 221649-038 Lisbon, Portugal,2 Instituto de Ciencia Aplicada e Tecnologia, Campus da FCUL, Campo Grande, 1749-016 Lisbon, Portugal,3 Department of Chemical Engineering, University of Loughborough, Loughborough LE11 3TU, United Kingdom,4 Universidade do Algarve, FERN, IBB-CBME, Campus de Gambelas, 8000-117 Faro, Portugal5

Received 26 October 2007/ Accepted 20 May 2008

Listeria monocytogenes is a food-borne pathogen capable of adhering to a range of surfaces utilized within the food industry, including stainless steel. The factors required for the attachment of this ubiquitous organism to abiotic surfaces are still relatively unknown. In silico analysis of the L. monocytogenes EGD genome identified a putative cell wall-anchored protein (Lmo0435 [BapL]), which had similarity to proteins involved in biofilm formation by staphylococci. An insertion mutation was constructed in L. monocytogenes to determine the influence of this protein on attachment to abiotic surfaces. The results show that the protein may contribute to the surface adherence of strains that possess BapL, but it is not an essential requirement for all L. monocytogenes strains. Several BapL-negative field isolates demonstrated an ability to adhere to abiotic surfaces equivalent to that of BapL-positive strains. BapL is not required for the virulence of L. monocytogenes in mice.

* Corresponding author. Mailing address: Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, United Kingdom. Phone: 44 116 252 2941. Fax: 44 116 252 5030. E-mail: pwa{at}le.ac.uk

{triangledown} Published ahead of print on 30 May 2008.

Applied and Environmental Microbiology, September 2008, p. 5451-5456, Vol. 74, No. 17
0099-2240/08/$08.00+0     doi:10.1128/AEM.02419-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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