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Applied and Environmental Microbiology, September 2008, p. 5571-5574, Vol. 74, No. 17
0099-2240/08/$08.00+0     doi:10.1128/AEM.00224-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

PCR Detection of Type I Polyketide Synthase Genes in Myxobacteria{triangledown} ,{dagger}

Hisayuki Komaki,1* Ryosuke Fudou,2 Takashi Iizuka,2 Daisuke Nakajima,3 Koei Okazaki,3 Daisuke Shibata,3 Makoto Ojika,4 and Shigeaki Harayama1

NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), Kisarazu, Chiba 292-0818, Japan,1 Ajinomoto Co., Inc., Kawasaki-ku, Kawasaki 210-8681, Japan,2 Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan,3 Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan4

Received 25 January 2008/ Accepted 30 June 2008

The diversity of type I modular polyketide synthase (PKS) was explored by PCR amplification of DNA encoding ketosynthase and acyltransferase domains in myxobacteria. The sequencing of the amplicons revealed that many PKS genes were distantly related to the published sequences. Thus, myxobacteria may be excellent resources for novel and diverse polyketides.

* Corresponding author. Mailing address: NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan. Phone: 81-438-20-5764. Fax: 81-438-52-5766. E-mail: hskomaki{at}nbrc.nite.go.jp

{triangledown} Published ahead of print on 7 July 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, September 2008, p. 5571-5574, Vol. 74, No. 17
0099-2240/08/$08.00+0     doi:10.1128/AEM.00224-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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