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Applied and Environmental Microbiology, September 2008, p. 5579-5582, Vol. 74, No. 17
0099-2240/08/$08.00+0     doi:10.1128/AEM.00995-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Autoaggregation of Xylella fastidiosa Cells Is Influenced by Type I and Type IV Pili{triangledown} ,{dagger}

Leonardo De La Fuente, Thomas J. Burr, and Harvey C. Hoch*

Department of Plant Pathology, Cornell University—New York State Agricultural Experiment Station, Geneva, New York

Received 23 April 2008/ Accepted 2 July 2008

Autoaggregation of widely dispersed Xylella fastidiosa cells into compact cell masses occurred over a period of hours following 7 to 11 days of growth in microfluidic chambers. Studies involving the use of mutants defective in polarly positioned type I (fimA-negative), type IV (pilB-negative), or both type I and IV (fimA- and pilO-negative) pili revealed the importance and role of pili in the autoaggregation process.

* Corresponding author. Mailing address: Department of Plant Pathology and Plant-Microbe Biology, Cornell University—New York State Agricultural Experiment Station, Geneva, NY 14456. Phone: (315) 787-2332. Fax: (315) 787-2389. E-mail: hch1{at}cornell.edu

{triangledown} Published ahead of print on 18 July 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, September 2008, p. 5579-5582, Vol. 74, No. 17
0099-2240/08/$08.00+0     doi:10.1128/AEM.00995-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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