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 Previous Article

Applied and Environmental Microbiology, September 2008, p. 5593-5597, Vol. 74, No. 17
0099-2240/08/$08.00+0     doi:10.1128/AEM.00303-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Selective Isolation and Rapid Identification of Members of the Genus Micromonospora{triangledown} ,{dagger}

Danheng Qiu,1,2 Jisheng Ruan,1 and Ying Huang1*

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China,1 Graduate University of Chinese Academy of Sciences, Beijing 100049, China2

Received 5 February 2008/ Accepted 30 June 2008

Improved methods for selective isolation of diverse actinomycetes of the genus Micromonospora and a genus-specific nested PCR for rapid identification of putative Micromonospora isolates were developed. The robustness of both the isolation and the identification approach was underpinned by phylogenetic analysis based on 16S rRNA gene sequences.

* Corresponding author. Mailing address: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. Phone: 86 (0) 10 6480 7311. Fax: 86 (0) 10 6480 7436. E-mail: huangy{at}im.ac.cn

{triangledown} Published ahead of print on 11 July 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, September 2008, p. 5593-5597, Vol. 74, No. 17
0099-2240/08/$08.00+0     doi:10.1128/AEM.00303-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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