This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Connor, M. R.
Right arrow Articles by Liao, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Connor, M. R.
Right arrow Articles by Liao, J. C.
Agricola
Right arrow Articles by Connor, M. R.
Right arrow Articles by Liao, J. C.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 2008, p. 5769-5775, Vol. 74, No. 18
0099-2240/08/$08.00+0     doi:10.1128/AEM.00468-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Engineering of an Escherichia coli Strain for the Production of 3-Methyl-1-Butanol{triangledown}

Michael R. Connor and James C. Liao*

Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, California 90095

Received 27 February 2008/ Accepted 23 July 2008

3-Methyl-1-butanol is a potential fuel additive or substitute. Previously this compound was identified in small quantities in yeast fermentation as one of the fusel alcohols. In this work, we engineered an Escherichia coli strain to produce 3-methyl-1-butanol from glucose via the host's amino acid biosynthetic pathways. Strain improvement with the removal of feedback inhibition and competing pathways increased the selectivity and productivity of 3-methyl-1-butanol. This work demonstrates the feasibility of production of 3-methyl-1-butanol as a biofuel and shows promise in using E. coli as a host for production.

* Corresponding author. Mailing address: University of California, Department of Chemical and Biomolecular Engineering, 5531 Boelter Hall, Los Angeles, CA 90095. Phone: (310) 825-1656. Fax: (310) 206-4107. E-mail: liaoj{at}ucla.edu

{triangledown} Published ahead of print on 1 August 2008.

Applied and Environmental Microbiology, September 2008, p. 5769-5775, Vol. 74, No. 18
0099-2240/08/$08.00+0     doi:10.1128/AEM.00468-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»