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Applied and Environmental Microbiology, September 2008, p. 5857-5861, Vol. 74, No. 18
0099-2240/08/$08.00+0     doi:10.1128/AEM.00435-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Purification, Molecular Cloning, and Enzymatic Properties of a Family 12 Endoglucanase (EG-II) from Fomitopsis palustris: Role of EG-II in Larch Holocellulose Hydrolysis{triangledown}

Tomoko Shimokawa,1* Hajime Shibuya,1 Masanobu Nojiri,1 Shigeki Yoshida,2 and Mitsuro Ishihara1

Department of Applied Microbiology, Forestry and Forest Products Research Institute, Matsunosato 1, Tsukuba, Ibaraki 305-8687,1 Institute of Applied Biochemistry, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan2

Received 22 February 2008/ Accepted 10 July 2008

A family 12 endoglucanase with a molecular mass of 23,926 Da (EG-II) from the brown-rot basidiomycete Fomitopsis palustris was purified and characterized. One of the roles of EG-II in wood degradation is thought to be to loosen the polysaccharide network in cell walls by disentangling hemicelluloses that are associated with cellulose.

* Corresponding author. Mailing address: Forestry and Forest Products Research Institute, Matsunosato 1, Tsukuba, Ibaraki 305-8687, Japan. Phone: 81-29-829-8281. Fax: 81-29-873-3797. E-mail: tshimo{at}ffpri.affrc.go.jp

{triangledown} Published ahead of print on 25 July 2008.

Applied and Environmental Microbiology, September 2008, p. 5857-5861, Vol. 74, No. 18
0099-2240/08/$08.00+0     doi:10.1128/AEM.00435-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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