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Applied and Environmental Microbiology, October 2008, p. 6026-6031, Vol. 74, No. 19
0099-2240/08/$08.00+0     doi:10.1128/AEM.00606-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Cryptosporidium Genotypes and Subtypes in Lambs and Goat Kids in Spain{triangledown}

Joaquín Quílez,1* Eucaris Torres,1 Rachel M. Chalmers,2 Stephen J. Hadfield,2 Emilio del Cacho,1 and Caridad Sánchez-Acedo1

Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, 50013 Zaragoza, Spain,1 United Kingdom Cryptosporidium Reference Unit, NPHS Microbiology Swansea, Singleton Hospital, Swansea SA2 8QA, United Kingdom2

Received 13 March 2008/ Accepted 6 July 2008

To provide information on the transmission dynamics of cryptosporidial infections in domestic small ruminants and the potential role of sheep and goats as a source for human cryptosporidiosis, Cryptosporidium-positive isolates from 137 diarrheic lambs and 17 goat kids younger than 21 days of age were examined by using genotyping and subtyping techniques. Fecal specimens were collected between 2004 and 2006 from 71 sheep and 7 goat farms distributed throughout Aragón (northeastern Spain). Cryptosporidium parvum was the only species identified by restriction analyses of PCR products from small-subunit rRNA genes from all 154 microscopy-positive isolates and the sequencing of a subset of 50 isolates. Sequence analyses of the glycoprotein (GP60) gene revealed extensive genetic diversity within the C. parvum strains in a limited geographical area, in which the isolates from lambs exhibited 11 subtypes in two subtype families (IId and IIa) and those from goat kids displayed four subtypes within the family IId. Most isolates (98%) belonged to the subtype family IId, whereas only three isolates belonged to the most widely distributed family, IIa. Three of the four most prevalent subtypes (IIdA17G1a, IIdA19G1, and IIdA18G1) were previously identified in humans, and five subtypes (IIdA14G1, IIdA15G1, IIdA24G1, IIdA25G1, and IIdA26G1) were novel subtypes. All IId subtypes were identical to each other in the nonrepeat region, except for subtypes IIdA17G1b and IIdA22G1, which differed by a single nucleotide polymorphism downstream of the trinucleotide repeats. These findings suggest that lambs and goat kids are an important reservoir of the zoonotic C. parvum subtype family IId for humans.

* Corresponding author. Mailing address: Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain. Phone: 34 976 762150. Fax: 34 976 761612. E-mail: jquilez{at}unizar.es

{triangledown} Published ahead of print on 11 July 2008.

Applied and Environmental Microbiology, October 2008, p. 6026-6031, Vol. 74, No. 19
0099-2240/08/$08.00+0     doi:10.1128/AEM.00606-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Xiao, L. (2009). Overview of Cryptosporidium Presentations at the 10th International Workshops on Opportunistic Protists. Eukaryot Cell 8: 429-436 [Full Text]  


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