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Applied and Environmental Microbiology, January 2008, p. 485-494, Vol. 74, No. 2
0099-2240/08/$08.00+0     doi:10.1128/AEM.01531-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Transcriptome Analysis of Lactococcus lactis in Coculture with Saccharomyces cerevisiae{triangledown}

Mathieu Maligoy, Myriam Mercade, Muriel Cocaign-Bousquet, and Pascal Loubiere*

UMR5504, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, CNRS, INRA, INSA, F-31400 Toulouse, France

Received 6 July 2007/ Accepted 30 October 2007

The study of microbial interactions in mixed cultures remains an important conceptual and methodological challenge for which transcriptome analysis could prove to be the essential method for improving our understanding. However, the use of whole-genome DNA chips is often restricted to the pure culture of the species for which the chips were designed. In this study, massive cross-hybridization was observed between the foreign cDNA and the specific Lactococcus lactis DNA chip. A very simple method is proposed to considerably decrease this nonspecific hybridization, consisting of adding the microbial partner's DNA. A correlation was established between the resulting cross-hybridization and the phylogenetic distance between the microbial partners. The response of L. lactis to the presence of Saccharomyces cerevisiae was analyzed during the exponential growth phase in fermentors under defined growth conditions. Although no differences between growth kinetics were observed for the pure and the mixed cultures of L. lactis, the mRNA levels of 158 genes were significantly modified. More particularly, a strong reorientation of pyrimidine metabolism was observed when L. lactis was grown in mixed cultures. These changes in transcript abundance were demonstrated to be regulated by the ethanol produced by the yeast and were confirmed by an independent method (quantitative reverse transcription-PCR).

* Corresponding author. Mailing address: INSA-LISBP, 135 Avenue de Rangueil, 31077 Toulouse cedex 4, France. Phone: 33 561 559 465. Fax: 33 561 559 400. E-mail: pascal.loubiere{at}insa-toulouse.fr

{triangledown} Published ahead of print on 9 November 2007.

Applied and Environmental Microbiology, January 2008, p. 485-494, Vol. 74, No. 2
0099-2240/08/$08.00+0     doi:10.1128/AEM.01531-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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