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Applied and Environmental Microbiology, January 2008, p. 516-525, Vol. 74, No. 2
0099-2240/08/$08.00+0     doi:10.1128/AEM.00990-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Complete Genomic Sequence of Bacteriophage {phi}EcoM-GJ1, a Novel Phage That Has Myovirus Morphology and a Podovirus-Like RNA Polymerase{triangledown}

Nidham Jamalludeen,1 Andrew M. Kropinski,2,3 Roger P. Johnson,2 Erika Lingohr,2 Josée Harel,4 and Carlton L. Gyles1*

Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada,1 Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario N1G 3W4, Canada,2 Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada,3 Faculté de Médecine Vétérinaire, Departement de Pathologie et Immunologie, Uníversité de Montréal, Saint-Hyacinthe, Quebec J2S 7C6, Canada4

Received 2 May 2007/ Accepted 12 November 2007

The complete genome of {phi}EcoM-GJ1, a lytic phage that attacks porcine enterotoxigenic Escherichia coli of serotype O149:H10:F4, was sequenced and analyzed. The morphology of the phage and the identity of the structural proteins were also determined. The genome consisted of 52,975 bp with a G+C content of 44% and was terminally redundant and circularly permuted. Seventy-five potential open reading frames (ORFs) were identified and annotated, but only 29 possessed homologs. The proteins of five ORFs showed homology with proteins of phages of the family Myoviridae, nine with proteins of phages of the family Podoviridae, and six with proteins of phages of the family Siphoviridae. ORF 1 encoded a T7-like single-subunit RNA polymerase and was preceded by a putative E. coli {sigma}70-like promoter. Nine putative phage promoters were detected throughout the genome. The genome included a tRNA gene of 95 bp that had a putative 18-bp intron. The phage morphology was typical of phages of the family Myoviridae, with an icosahedral head, a neck, and a long contractile tail with tail fibers. The analysis shows that {phi}EcoM-GJ1 is unique, having the morphology of the Myoviridae, a gene for RNA polymerase, which is characteristic of phages of the T7 group of the Podoviridae, and several genes that encode proteins with homology to proteins of phages of the family Siphoviridae.

* Corresponding author. Mailing address: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada. Phone: (519) 824-4120, ext. 54715. Fax: (519) 824-5930. E-mail: cgyles{at}uoguelph.ca

{triangledown} Published ahead of print on 26 November 2007.

Applied and Environmental Microbiology, January 2008, p. 516-525, Vol. 74, No. 2
0099-2240/08/$08.00+0     doi:10.1128/AEM.00990-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhang, Y., Jiao, N. (2009). Roseophage RDJL{Phi}1, Infecting the Aerobic Anoxygenic Phototrophic Bacterium Roseobacter denitrificans OCh114. Appl. Environ. Microbiol. 75: 1745-1749 [Abstract] [Full Text]  


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