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Applied and Environmental Microbiology, January 2008, p. 531-534, Vol. 74, No. 2
0099-2240/08/$08.00+0     doi:10.1128/AEM.01710-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluation of Escherichia coli Host Strain CB390 for Simultaneous Detection of Somatic and F-Specific Coliphages

Department of Microbiology, Biology Faculty, University of Barcelona, 645 Diagonal Avenue, 08028 Barcelona, Spain

Received 25 July 2007/ Accepted 7 November 2007

Escherichia coli WG5, the strain recommended by the International Organization for Standardization (ISO) to detect somatic coliphages, was transformed to F⁺ by introducing the plasmid Famp, which rendered it capable of simultaneously detecting both somatic and F-specific coliphages. Indeed, this strain, CB390, proved as effective in detecting similar numbers of phages as the sum of somatic and F-specific bacteriophages detected by the host strains recommended by both the ISO and the U.S. Environmental Protection Agency standardized methods.

Published ahead of print on 16 November 2007.