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Applied and Environmental Microbiology, January 2008, p. 531-534, Vol. 74, No. 2
0099-2240/08/$08.00+0     doi:10.1128/AEM.01710-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluation of Escherichia coli Host Strain CB390 for Simultaneous Detection of Somatic and F-Specific Coliphages{triangledown}

Carolina Guzmán, Laura Mocé-Llivina, Francisco Lucena, and Juan Jofre*

Department of Microbiology, Biology Faculty, University of Barcelona, 645 Diagonal Avenue, 08028 Barcelona, Spain

Received 25 July 2007/ Accepted 7 November 2007

Escherichia coli WG5, the strain recommended by the International Organization for Standardization (ISO) to detect somatic coliphages, was transformed to F+ by introducing the plasmid Famp, which rendered it capable of simultaneously detecting both somatic and F-specific coliphages. Indeed, this strain, CB390, proved as effective in detecting similar numbers of phages as the sum of somatic and F-specific bacteriophages detected by the host strains recommended by both the ISO and the U.S. Environmental Protection Agency standardized methods.

* Corresponding author. Mailing address: Department of Microbiology, Biology Faculty, University of Barcelona, 645 Diagonal Avenue, 08028 Barcelona, Spain. Phone: 34-93-4021487. Fax: 34-93-4039047. E-mail: jjofre{at}ub.edu

{triangledown} Published ahead of print on 16 November 2007.

Applied and Environmental Microbiology, January 2008, p. 531-534, Vol. 74, No. 2
0099-2240/08/$08.00+0     doi:10.1128/AEM.01710-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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