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Applied and Environmental Microbiology, January 2008, p. 543-546, Vol. 74, No. 2
0099-2240/08/$08.00+0     doi:10.1128/AEM.01039-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Detection of Murine Norovirus 1 by Using Plaque Assay, Transfection Assay, and Real-Time Reverse Transcription-PCR before and after Heat Exposure{triangledown}

Leen Baert,1 Christiane E. Wobus,2,{dagger} Els Van Coillie,3 Larissa B. Thackray,2 Johan Debevere,1 and Mieke Uyttendaele1*

Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation, Ghent University, Coupure Links 653, 9000 Ghent,1 Institute for Agricultural and Fisheries Research, Technology and Food Unit, Brusselsesteenweg 370, 9090 Melle, Belgium,3 Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri2

Received 10 May 2007/ Accepted 21 October 2007

The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80°C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.

* Corresponding author. Mailing address: Ghent University, Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation, Coupure links 653, 9000 Ghent, Belgium. Phone: 32 9 264 61 78. Fax: 32 9 225 55 10. E-mail: Mieke.Uyttendaele{at}UGent.be

{triangledown} Published ahead of print on 16 November 2007.

{dagger} Present address: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI.

Applied and Environmental Microbiology, January 2008, p. 543-546, Vol. 74, No. 2
0099-2240/08/$08.00+0     doi:10.1128/AEM.01039-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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