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Applied and Environmental Microbiology, January 2008, p. 547-549, Vol. 74, No. 2
0099-2240/08/$08.00+0     doi:10.1128/AEM.01862-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Characterization of the Structural Gene Encoding Nisin F, a New Lantibiotic Produced by a Lactococcus lactis subsp. lactis Isolate from Freshwater Catfish (Clarias gariepinus){triangledown}

M. de Kwaadsteniet, K. ten Doeschate, and L. M. T. Dicks*

Department of Microbiology, Stellenbosch University, Stellenbosch, South Africa

Received 10 August 2007/ Accepted 12 November 2007

Lactococcus lactis F10, isolated from freshwater catfish, produces a bacteriocin (BacF) active against Staphylococcus aureus, Staphylococcus carnosus, Lactobacillus curvatus, Lactobacillus plantarum, and Lactobacillus reuteri. The operon encoding BacF is located on a plasmid. Sequencing of the structural gene revealed no homology to other nisin genes. Nisin F is described.

* Corresponding author. Mailing address: Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland 7602, Stellenbosch, South Africa. Phone: 27-21-808 5849. Fax: 27-21-808 5846. E-mail: lmtd{at}sun.ac.za

{triangledown} Published ahead of print on 26 November 2007.

Applied and Environmental Microbiology, January 2008, p. 547-549, Vol. 74, No. 2
0099-2240/08/$08.00+0     doi:10.1128/AEM.01862-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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