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Applied and Environmental Microbiology, October 2008, p. 6216-6222, Vol. 74, No. 20
0099-2240/08/$08.00+0     doi:10.1128/AEM.00963-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Engineering of a Glycerol Utilization Pathway for Amino Acid Production by Corynebacterium glutamicum{triangledown}

Doris Rittmann,1 Steffen N. Lindner,2 and Volker F. Wendisch2*

Institute of Biotechnology I, Research Center Juelich, Juelich, Germany,1 Institute of Molecular Microbiology and Biotechnology, Westfalian Wilhelms University Muenster, Muenster, Germany2

Received 28 April 2008/ Accepted 15 August 2008

The amino acid-producing organism Corynebacterium glutamicum cannot utilize glycerol, a stoichiometric by-product of biodiesel production. By heterologous expression of Escherichia coli glycerol utilization genes, C. glutamicum was engineered to grow on glycerol. While expression of the E. coli genes for glycerol kinase (glpK) and glycerol 3-phosphate dehydrogenase (glpD) was sufficient for growth on glycerol as the sole carbon and energy source, additional expression of the aquaglyceroporin gene glpF from E. coli increased growth rate and biomass formation. Glutamate production from glycerol was enabled by plasmid-borne expression of E. coli glpF, glpK, and glpD in C. glutamicum wild type. In addition, a lysine-producing C. glutamicum strain expressing E. coli glpF, glpK, and glpD was able to produce lysine from glycerol as the sole carbon substrate as well as from glycerol-glucose mixtures.

* Corresponding author. Mailing address: Institute of Molecular Microbiology and Biotechnology, Westfalian Wilhelms University Muenster, Corrensstr. 3, D-48149 Muenster, Germany. Phone: 49-251-833 9827. Fax: 49-251-833 8388. E-mail: wendisch{at}uni-muenster.de

{triangledown} Published ahead of print on 29 August 2008.

Applied and Environmental Microbiology, October 2008, p. 6216-6222, Vol. 74, No. 20
0099-2240/08/$08.00+0     doi:10.1128/AEM.00963-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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