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Applied and Environmental Microbiology, October 2008, p. 6223-6229, Vol. 74, No. 20
0099-2240/08/$08.00+0     doi:10.1128/AEM.01351-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

First Cultivation and Ecological Investigation of a Bacterium Affiliated with the Candidate Phylum OP5 from Hot Springs {triangledown}

Koji Mori,1* Michinari Sunamura,2 Katsunori Yanagawa,2 Jun-ichiro Ishibashi,3 Youko Miyoshi,3 Takao Iino,1 Ken-ichiro Suzuki,1 and Tetsuro Urabe2

NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan,1 Department of Earth and Planetary Science, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan,2 Faculty of Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan3

Received 17 June 2008/ Accepted 19 August 2008

The phylogenetic group termed OP5 was originally discovered in the Yellowstone National Park hot spring and proposed as an uncultured phylum; the group was afterwards analyzed by applying culture-independent approaches. Recently, a novel thermophilic chemoheterotrophic filamentous bacterium was obtained from a hot spring in Japan that was enriched through various isolation procedures. Phylogenetic analyses of the isolate have revealed that it is closely related to the OP5 phylum that has mainly been constructed with the environmental clones retrieved from thermophilic and mesophilic anaerobic environments. It appears that the lineage is independent at the phylum level in the domain Bacteria. Therefore, we designed a primer set for the 16S rRNA gene to specifically target the OP5 phylum and performed quantitative field analysis by using the real-time PCR method. Thus, the 16S rRNA gene of the OP5 phylum was detected in some hot-spring samples with the relative abundance ranging from 0.2% to 1.4% of the prokaryotic organisms detected. The physiology of the above-mentioned isolate and the related environmental clones indicated that they are scavengers contributing to the sulfur cycle in nature.

* Corresponding author. Mailing address: NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan. Phone: 81-438-20-5763. Fax: 81-438-52-2329. E-mail: mori{at}nbrc.nite.go.jp

{triangledown} Published ahead of print on 5 September 2008.

Applied and Environmental Microbiology, October 2008, p. 6223-6229, Vol. 74, No. 20
0099-2240/08/$08.00+0     doi:10.1128/AEM.01351-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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