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Applied and Environmental Microbiology, October 2008, p. 6405-6416, Vol. 74, No. 20
0099-2240/08/$08.00+0     doi:10.1128/AEM.00197-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

The Genome of Polaromonas sp. Strain JS666: Insights into the Evolution of a Hydrocarbon- and Xenobiotic-Degrading Bacterium, and Features of Relevance to Biotechnology{triangledown} ,{dagger}

Timothy E. Mattes,1* Anne K. Alexander,1 Paul M. Richardson,2 A. Christine Munk,3 Cliff S. Han,3 Paul Stothard,4 and Nicholas V. Coleman5

Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa, Iowa City, Iowa 52242,1 Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, California 94598,2 Los Alamos National Laboratory, Los Alamos, New Mexico 87545,3 Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5,4 School of Molecular and Microbial Biosciences, Building G08, University of Sydney, Sydney, New South Wales 2006, Australia5

Received 22 January 2008/ Accepted 12 August 2008

Polaromonas sp. strain JS666 can grow on cis-1,2-dichloroethene (cDCE) as a sole carbon and energy source and may be useful for bioremediation of chlorinated solvent-contaminated sites. Analysis of the genome sequence of JS666 (5.9 Mb) shows a bacterium well adapted to pollution that carries many genes likely to be involved in hydrocarbon and xenobiotic catabolism and metal resistance. Clusters of genes coding for haloalkane, haloalkanoate, n-alkane, alicyclic acid, cyclic alcohol, and aromatic catabolism were analyzed in detail, and growth on acetate, catechol, chloroacetate, cyclohexane carboxylate, cyclohexanol, ferulate, heptane, 3-hydroxybenzoate, hydroxyquinol, gentisate, octane, protocatechuate, and salicylate was confirmed experimentally. Strain JS666 also harbors diverse putative mobile genetic elements, including retrons, inteins, a miniature inverted-repeat transposable element, insertion sequence transposases from 14 families, eight genomic islands, a Mu family bacteriophage, and two large (338- and 360-kb) plasmids. Both plasmids are likely to be self-transferable and carry genes for alkane, alcohol, aromatic, and haloacid metabolism. Overall, the JS666 genome sequence provides insights into the evolution of pollutant-degrading bacteria and provides a toolbox of catabolic genes with utility for biotechnology.

* Corresponding author. Mailing address: Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa, Iowa City, IA 52242. Phone: (319) 335-5065. Fax: (319) 335-5660. E-mail: tim-mattes{at}uiowa.edu

{triangledown} Published ahead of print on 22 August 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, October 2008, p. 6405-6416, Vol. 74, No. 20
0099-2240/08/$08.00+0     doi:10.1128/AEM.00197-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jennings, L. K., Chartrand, M. M. G., Lacrampe-Couloume, G., Lollar, B. S., Spain, J. C., Gossett, J. M. (2009). Proteomic and Transcriptomic Analyses Reveal Genes Upregulated by cis-Dichloroethene in Polaromonas sp. Strain JS666. Appl. Environ. Microbiol. 75: 3733-3744 [Abstract] [Full Text]  


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