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Applied and Environmental Microbiology, October 2008, p. 6457-6460, Vol. 74, No. 20
0099-2240/08/$08.00+0     doi:10.1128/AEM.00895-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Isolation and Identification of Bifidobacteriaceae from Human Saliva{triangledown}

David Beighton,1,2* Steven C. Gilbert,2 Douglas Clark,1 Maria Mantzourani,1 Mustafa al-Haboubi,1 Farida Ali,1 Elizabeth Ransome,1 Nicholas Hodson,1 Michael Fenlon,1 Liana Zoitopoulos,1 and Jennifer Gallagher1

Infection Research Group, Dental Institute, King's College London, London, United Kingdom SE1 9RT,1 Biomedical Research Centre, Guy's and St. Thomas' Hospital Foundation Trust, London, United Kingdom SE1 9RT2

Received 20 April 2008/ Accepted 11 August 2008

Bifidobacteriaceae were isolated from saliva and infected dentine by using a mupirocin-based selective medium. Of the saliva samples, 94% harbored bifids. The mean concentration (± the standard error) was 4.46 (±0.12) log10(CFU per ml + 1), and the predominant isolates were Bifidobacterium dentium, B. longum, Scardovia inopinata, Parascardovia denticolens, and Alloscardovia omnicolens.

* Corresponding author. Mailing address: Infection Research Group, Dental Institute, King's College London, London, United Kingdom SE1 9RT. Phone: 44-207-188-7465. Fax: 44-207-188-7466. E-mail: david.beighton{at}kcl.ac.uk

{triangledown} Published ahead of print on 22 August 2008.

Applied and Environmental Microbiology, October 2008, p. 6457-6460, Vol. 74, No. 20
0099-2240/08/$08.00+0     doi:10.1128/AEM.00895-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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