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Applied and Environmental Microbiology, October 2008, p. 6461-6464, Vol. 74, No. 20
0099-2240/08/$08.00+0     doi:10.1128/AEM.01424-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Development of a Transposon Mutagenesis System in the Oral Spirochete Treponema denticola{triangledown}

Yu Yang,1 Philip E. Stewart,2 Xiaoguang Shi,1 and Chunhao Li1*

Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York 14214,1 Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 598402

Received 25 June 2008/ Accepted 11 August 2008

Here, we report successful transposon mutagenesis in the oral spirochete Treponema denticola. A modified Himar1 transposon, including a new antibiotic selection cassette for T. denticola, generated mutations affecting cell division, transport, and chemotaxis, among other processes. This random mutagenesis system should facilitate research on the biology and pathogenesis of this spirochete, which is associated with human periodontal diseases.

* Corresponding author. Mailing address: Department of Oral Biology, SUNY at Buffalo, 3435 Main St., Buffalo, NY 14214-3092. Phone: (716) 829-6014. Fax: (716) 829-3942. E-mail: cli9{at}buffalo.edu

{triangledown} Published ahead of print on 22 August 2008.

Applied and Environmental Microbiology, October 2008, p. 6461-6464, Vol. 74, No. 20
0099-2240/08/$08.00+0     doi:10.1128/AEM.01424-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rollefson, J. B., Levar, C. E., Bond, D. R. (2009). Identification of Genes Involved in Biofilm Formation and Respiration via Mini-Himar Transposon Mutagenesis of Geobacter sulfurreducens. J. Bacteriol. 191: 4207-4217 [Abstract] [Full Text]  


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