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Applied and Environmental Microbiology, October 2008, p. 6465-6469, Vol. 74, No. 20
0099-2240/08/$08.00+0     doi:10.1128/AEM.01459-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

TaqMan-Based Real-Time PCR Method for Detection of Yersinia pseudotuberculosis in Food{triangledown}

S. Thisted Lambertz,1* C. Nilsson,1 and S. Hallanvuo2

Research and Development Department, National Food Administration, Uppsala, Sweden,1 Environmental and Food Research Laboratory (TavastLab), Municipal Joint Union for Public Health in Hämeenlinna Region, Hämeenlinna, Finland2

Received 30 June 2008/ Accepted 21 August 2008

A sensitive and specific assay for detection of food-borne pathogenic Yersinia pseudotuberculosis was developed. The primer-probe set was designed to target a 157-bp sequence of the chromosomally located gene ail. The complete method, including an internal amplification control, was evaluated for several different food items.

* Corresponding author. Mailing address: National Food Administration, Research and Development Department, P.O. Box 622, SE-751 26 Uppsala, Sweden. Phone: 46 (0)18 17 55 62. Fax: 46 (0)18 17 14 94. E-mail: sula{at}slv.se

{triangledown} Published ahead of print on 29 August 2008.

Applied and Environmental Microbiology, October 2008, p. 6465-6469, Vol. 74, No. 20
0099-2240/08/$08.00+0     doi:10.1128/AEM.01459-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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