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Applied and Environmental Microbiology, October 2008, p. 6476-6480, Vol. 74, No. 20
0099-2240/08/$08.00+0     doi:10.1128/AEM.01082-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification of Critical Members in a Sulfidogenic Benzene-Degrading Consortium by DNA Stable Isotope Probing{triangledown}

A. R. Oka,1 C. D. Phelps,1 L. M. McGuinness,2 A. Mumford,1 L. Y. Young,1 and L. J. Kerkhof2*

Department of Environmental Sciences,1 Institute of Marine and Coastal Sciences, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 089012

Received 14 May 2008/ Accepted 14 August 2008

Stable isotope probing (SIP) was used to identify the active members in a benzene-degrading sulfidogenic consortium. SIP-terminal restriction fragment length polymorphism analysis indicated that a 270-bp peak incorporated the majority of the 13C label and is a sequence closely related to that of clone SB-21 (GenBank accession no. AF029045). This target may be an important biomarker for anaerobic benzene degradation in the field.

* Corresponding author. Mailing address: Dept. of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 71 Dudley Road, New Brunswick, NJ 08901. Phone: (732) 932-6555, ext. 335. Fax: (732) 932-8520. E-mail: lkerkhof{at}rutgers.edu

{triangledown} Published ahead of print on 29 August 2008.

Applied and Environmental Microbiology, October 2008, p. 6476-6480, Vol. 74, No. 20
0099-2240/08/$08.00+0     doi:10.1128/AEM.01082-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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