This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Burlakoti, R. R.
Right arrow Articles by Adhikari, T. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Burlakoti, R. R.
Right arrow Articles by Adhikari, T. B.
Agricola
Right arrow Articles by Burlakoti, R. R.
Right arrow Articles by Adhikari, T. B.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2008, p. 6513-6520, Vol. 74, No. 21
0099-2240/08/$08.00+0     doi:10.1128/AEM.01580-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Comparative Mycotoxin Profiles of Gibberella zeae Populations from Barley, Wheat, Potatoes, and Sugar Beets{triangledown}

Rishi R. Burlakoti, Shaukat Ali, Gary A. Secor, Stephen M. Neate, Marcia P. McMullen, and Tika B. Adhikari*

Department of Plant Pathology, North Dakota State University, Fargo, North Dakota 58105

Received 10 July 2008/ Accepted 28 August 2008

Gibberella zeae is one of the most devastating pathogens of barley and wheat in the United States. The fungus also infects noncereal crops, such as potatoes and sugar beets, and the genetic relationships among barley, wheat, potato, and sugar beet isolates indicate high levels of similarity. However, little is known about the toxigenic potential of G. zeae isolates from potatoes and sugar beets. A total of 336 isolates of G. zeae from barley, wheat, potatoes, and sugar beets were collected and analyzed by TRI (trichothecene biosynthesis gene)-based PCR assays. To verify the TRI-based PCR detection of genetic markers by chemical analysis, 45 representative isolates were grown in rice cultures for 28 days and 15 trichothecenes and 2 zearalenone (ZEA) analogs were quantified using gas chromatography-mass spectrometry. TRI-based PCR assays revealed that all isolates had the deoxynivalenol (DON) marker. The frequencies of isolates with the 15-acetyl-deoxynivalenol (15-ADON) marker were higher than those of isolates with the 3-acetyl-deoxynivalenol (3-ADON) marker among isolates from all four crops. Fusarium head blight (FHB)-resistant wheat cultivars had little or no influence on the diversity of isolates associated with the 3-ADON and 15-ADON markers. However, the frequency of isolates with the 3-ADON marker among isolates from the Langdon, ND, sampling site was higher than those among isolates from the Carrington and Minot, ND, sites. In chemical analyses, DON, 3-ADON, 15-ADON, b-ZEA, and ZEA were detected. All isolates produced DON (1 to 782 µg/g) and ZEA (1 to 623 µg/g). These findings may be useful for monitoring mycotoxin contamination and for formulating FHB management strategies for these crops.

* Corresponding author. Mailing address: Department of Plant Pathology, North Dakota State University, Fargo, ND 58105. Phone: (701) 231-7079. Fax: (701) 231-7851. E-mail: tika.adhikari{at}ndsu.edu

{triangledown} Published ahead of print on 12 September 2008.

Applied and Environmental Microbiology, November 2008, p. 6513-6520, Vol. 74, No. 21
0099-2240/08/$08.00+0     doi:10.1128/AEM.01580-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»