This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van de Werken, H. J. G.
Right arrow Articles by Kengen, S. W. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van de Werken, H. J. G.
Right arrow Articles by Kengen, S. W. M.
Agricola
Right arrow Articles by van de Werken, H. J. G.
Right arrow Articles by Kengen, S. W. M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2008, p. 6720-6729, Vol. 74, No. 21
0099-2240/08/$08.00+0     doi:10.1128/AEM.00968-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Hydrogenomics of the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus{triangledown} ,{dagger}

Harmen J. G. van de Werken,1,{ddagger} Marcel R. A. Verhaart,1,{ddagger} Amy L. VanFossen,2,{ddagger} Karin Willquist,3 Derrick L. Lewis,2 Jason D. Nichols,2 Heleen P. Goorissen,1 Emmanuel F. Mongodin,4 Karen E. Nelson,5 Ed W. J. van Niel,3 Alfons J. M. Stams,1 Donald E. Ward,6 Willem M. de Vos,1 John van der Oost,1 Robert M. Kelly,2 and Servé W. M. Kengen1*

Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands,1 Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695-7905,2 Applied Microbiology, Lund Institute of Technology, Lund University, SE-221 00 Lund, Sweden,3 Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland 21201,4 The J. Craig Venter Institute, 9712 Medical Center Drive, Rockville, Maryland 20850,5 Genencor International, a Danisco Company, 925 Page Mill Road, Palo Alto, California 943046

Received 29 April 2008/ Accepted 27 August 2008

Caldicellulosiruptor saccharolyticus is an extremely thermophilic, gram-positive anaerobe which ferments cellulose-, hemicellulose- and pectin-containing biomass to acetate, CO2, and hydrogen. Its broad substrate range, high hydrogen-producing capacity, and ability to coutilize glucose and xylose make this bacterium an attractive candidate for microbial bioenergy production. Here, the complete genome sequence of C. saccharolyticus, consisting of a 2,970,275-bp circular chromosome encoding 2,679 predicted proteins, is described. Analysis of the genome revealed that C. saccharolyticus has an extensive polysaccharide-hydrolyzing capacity for cellulose, hemicellulose, pectin, and starch, coupled to a large number of ABC transporters for monomeric and oligomeric sugar uptake. The components of the Embden-Meyerhof and nonoxidative pentose phosphate pathways are all present; however, there is no evidence that an Entner-Doudoroff pathway is present. Catabolic pathways for a range of sugars, including rhamnose, fucose, arabinose, glucuronate, fructose, and galactose, were identified. These pathways lead to the production of NADH and reduced ferredoxin. NADH and reduced ferredoxin are subsequently used by two distinct hydrogenases to generate hydrogen. Whole-genome transcriptome analysis revealed that there is significant upregulation of the glycolytic pathway and an ABC-type sugar transporter during growth on glucose and xylose, indicating that C. saccharolyticus coferments these sugars unimpeded by glucose-based catabolite repression. The capacity to simultaneously process and utilize a range of carbohydrates associated with biomass feedstocks is a highly desirable feature of this lignocellulose-utilizing, biofuel-producing bacterium.

* Corresponding author. Mailing address: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands. Phone: 31-317-483737. Fax: 31-317-483829. E-mail: serve.kengen{at}wur.nl

{triangledown} Published ahead of print on 5 September 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} H.J.G.V.D.W., M.R.A.V., and A.L.V. contributed equally to this work.

Applied and Environmental Microbiology, November 2008, p. 6720-6729, Vol. 74, No. 21
0099-2240/08/$08.00+0     doi:10.1128/AEM.00968-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kataeva, I. A., Yang, S.-J., Dam, P., Poole, F. L. II, Yin, Y., Zhou, F., Chou, W.-c., Xu, Y., Goodwin, L., Sims, D. R., Detter, J. C., Hauser, L. J., Westpheling, J., Adams, M. W. W. (2009). Genome Sequence of the Anaerobic, Thermophilic, and Cellulolytic Bacterium "Anaerocellum thermophilum" DSM 6725. J. Bacteriol. 191: 3760-3761 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»